M1 macrophage polarization assay

Macrophage Therapeutics Development Platform at Creative Biolabs can generate macrophages by treating isolated bone marrow-derived or peritoneal macrophages with specific stimuli, including secreting inflammatory cytokines, such as tumor necrosis factor–α (TNF-α), interferon-gamma (IFN-γ), and interleukins (IL-1, IL-6, IL-8), and bacterial components such as lipopolysaccharide (LPS), growth factors including granulocyte-macrophage colony-stimulating factor (GM-CSF), or matrix metalloproteinases (MMPs). Moreover, based on surface markers and the distinctly produced cytokines, we also provide the validation of each polarized macrophage. Enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), flow cytometry (FC) could be conducted to analyze surface markers and cytokines expression upon request. Our polarization protocols are designed to achieve a stable, optimal, and effective regimen for in vitro induction to drive antigen-specific immune responses. Our M1 macrophage polarization assay allows the assessment of cytokine profiles, cell surface receptor expression, scavenging functions, and the ability to activate or suppress T-cell proliferation.