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Therapeutic Fc Characterization
Therapeutic antibody development and production are significant components of the biopharmaceutical sector and have had a significant positive impact on public health. To be clinically effective, therapeutic antibodies must perform two different types of functions: (i) target-specific binding via variable domains, and (ii) immune-mediated effector function via interaction of Fc (crystalllizable fragment) domains with cell receptors and complement proteins. By attaching to Fc-receptors (FcR) on immune cells or repairing complement, antibodies and antibody-based therapies have the potential to enhance immune function. The Fc region of the mAb must be described for IND and BLA submissions in accordance with the ICH Q6B and EMA mAb criteria. Therapeutic Fc characterisation was developed to promote the development of biobetter or biosimilar treatments after focusing on the development of antibody therapeutics for many years. In particular, we offer services for Fc engineering to control antibody function as well as cell-based functional tests for assessing antibody effector activities.
Ad fiber knob CD40 Miniantibody
A fusion protein made of two different scFv segments, Recombinant anti-Ad fiber knob CD40 miniantibody has anti-Ad fiber knob and anti-CD40 specificity. A flexible hinge region connects one scFv of an anti-Ad fiber knob antibody variable domain to another scFv of an anti-CD40 antibody variable domain. Compared to typical IgGs, this BsAb structure has a smaller size and greater penetration for clinical diagnosis and maybe therapy. Adenoviral vectors can be retargeted by this BsAb to tumor cells. It can be applied in cancer gene therapy to deliver a therapeutic gene to tumor cells with the goal of efficient and targeted cell death.
Bioinformatic solutions for cancer
Global researchers have amassed extensive experience in bioinformatics analysis to support whole genome sequencing (WGS), whole exome sequencing (WES), targeted sequencing, whole transcriptome sequencing (WTS), and immune repertoire sequencing thanks to years of research and development experience in the field of next-generation sequencing (NGS). We are able to provide premium custom bioinformatics analysis services to satisfy each and every one of our clients' particular needs. The development of NGS platforms places an increasing burden on statistical techniques and bioinformatic tools to handle and analyze the massive volumes of data. NGS technologies are becoming more and more crucial in the domains of oncology and immunology due to their cost-effectiveness, remarkable sequencing speed, high resolution, and accuracy in genomic analysis. For medical diagnosis and therapy, the combined power of NGS and bioinformatics solutions for cancer is essential.
Human Skin Model
Animal testing, which has been vehemently condemned by animal welfare organizations, is the principal method used to test the safety of cosmetics. In vitro cell studies are typically used to determine whether cosmetics are effective, however it can be challenging to accurately represent the actual world considering how intricate human skin tissue is. A global trend in recent decades has been the development of alternate methods for assessing genotoxicity and skin irritancy. Our created ex vivo human skin models are derived from actual human skin tissue to provide the most accurate test findings and a better representation of human biology. According to reports, the human skin model can be used to assess a number of skin conditions, including atopic dermatitis (AD), skin permeation, and abnormalities of the skin barrier. Our human skin models preserve the natural skin's structure, including cell population and dermal matrix, using the latest models and technologies. In order to evaluate the effectiveness of each cosmetic ingredient, our model can mimic several kinds of skin bacteria or skin microbiomes. Additionally, our model is able to simulate the actual state of cosmetics applied to the skin as well as translate subjectively perceived efficacy into particular data output, making it a more objective, scientific, and reliable evaluation model for cosmetic efficacy evaluation.
PreciAbā¢ Platform
In the last ten years, computer science and technology have advanced significantly, impacting every part of our lives. Since the late 1990s, computational biology has grown to play a significant role in the area of biology's new technologies. Scientists are now able to obtain structural information with greater detail thanks to advances in computer hardware development, such as the introduction of supercomputers and the programming language for computational biology. This information is crucial for understanding the various functions and mechanisms of biological molecules, but it also holds the key to the development of molecules with new or improved functions. B lymphocytes create the binding proteins known as antibodies to protect an organism from infections and foreign macromolecules. They are frequently utilized as medicinal medicines, diagnostic tools, and research tools. Antibody molecules are distinguished by their extreme diversity and recognition specificity. The development of phage display technology has made it possible to choose antibodies to any antigen with a suitable binding affinity, but antibody characteristics still need to be further improved for their application, particularly for therapy. When accessible, finely tuned antibody structures, including the structure of the antibody-antigen complex, are particularly helpful for designing and engineering antibodies. Key phases of antibody creation are detailed in our PreciAbā¢ Platform.
Single Cell Omics for CNAS
Recent years have seen the application of single cell omics to the study of cancer, including single-cell genomes, epigenomes, transcriptomes, proteomes, and multi-omics. In the study of cancer evolution, fascinating findings have been made on treatment resistance and the mysteries of the tumor microenvironment. Single-cell genome sequencing has recently been discovered to be extremely useful for the detection of somatic mutations, particularly during the evolution of tumors. DNA copy number abnormalities (CNAS) or an abnormally high number of chromosomes are common in cancer genomes. A discontinuous model of copy number evolution may also be important in other solid tumors, according to preliminary evidence from researchers utilizing single-cell gene sequencing in diseases such prostate, colon, liver, and lung cancer. The clinical diagnosis and management of cancer patients, as well as how evolutionarily we interpret the dynamics of tumor growth, are all significantly affected by this approach. For more: single cell Omics for CNAS
Microfluidic Food Safety
The frequent occurrence of chemical (pesticide residues, heavy metals, and illegal additives) and biological (foodborne pathogens like Bacillus cereus, Listeria monocytogenes, Campylobacter jejuni, Sonne dysentery bacillus, E. coli, and Salmonella typhimurium) contaminations has made food safety a topic of international concern. As a result, food safety testing and analysis are crucial tools for preventing food contamination and resolving food safety issues. Historically, instrumental analysis has served as the foundation for most detection technologies. Even if it is accurate and trustworthy, instrumental analysis has some drawbacks, such as pricey equipment, a lengthy cycle, high material consumption, difficult operation, and low sensitivity. To meet the demands of on-site, real-time, quick, and portable food detection, it is imperative to design a novel analysis approach. Microfluidic Analysis in Food Safety The integration of sample pretreatment, separation, and detection into a chip with a few square centimeters has made microfluidic chip technology a hot topic in recent years. This allows sample pretreatment and follow-up analysis to be miniaturized, automated, integrated, and portable. It has been widely employed in the detection of pesticide residue, pathogenic bacteria, heavy metals, and food additives due to its benefits of low sample consumption, quick detection, simple operation, multi-functional integration, small size, and portability. For instance, a paper-based microfluidic device was created to detect leftover aminoglycoside antibiotics, Ag+, and Hg2+ simultaneously in food.
Qualify SARS-CoV-2 Vaccine
Infection can be prevented with vaccination, but it can also be treated through vaccination. Since the majority of potential vaccines fail in the preclinical and initial phase of research, vaccine development is a difficult, expensive, and dangerous process. Due to the intricacy of vaccine manufacture, it is crucial to completely comprehend the variables that will impact the formulation's safety, effectiveness, and stability. The selection of unfavorable conditions, or even a failure of safety, efficacy, or stability, could result from a lack of understanding of the elements that could have a negative impact on vaccine formulations and cause a project's delay or cancellation. As a result, when developing a vaccine, the evaluation of vaccine quality is crucial. It comprises assessing stabilizers, studying the interactions between antigen and adjuvant, evaluating product contact materials (such sterile filter membranes), and performing real-time and expedited stability monitoring. For more: SARS-CoV-2 vaccine qualification
Protein Modification Detection
Protein modification is the process of modifying proteins chemically either during or after translation. By adding functional groups to the protein, such as phosphate, acetate, amido, or methyl groups, protein modification detection expand the functional diversity of the proteome and have an impact on nearly every aspect of normal cell biology and disease. The regulation of gene expression, protein interactions, protein degradation, signaling, and regulatory mechanisms, as well as many other biological activities, depend on protein modification. The discovery of ribosomal proteomics has made it possible to modify ribosomal proteins, which represents a breakthrough in understanding the mystery of ribosomal protein function and offers a novel therapy approach for ribosome-related disorders. The properties of protein modification, including modification categories and modification sites, are crucial for cell biology, disease detection, and disease preventive studies as precision medicine advances.
Half-life Assay by Proteomic Approaches
The protein complement expressed by an organism's genome, or in the case of multicellular organisms, the protein complement produced by tissue or differentiated cells, has been characterized as the proteome. Half-life assay by proteomic approach can be a database repository, a biological assay, or a tool for biological study. Although mass spectrometry is an effective tool for many different protein investigations, its use is primarily limited to qualitative methods. In response to the recent realization of the importance of quantitation in proteomics, mass spectrometric techniques based on stable isotope quantitation, such as stable isotope labeling in cell culture (SILAC), were developed. SILAC showed great promise for the simultaneous and automated identification and quantitation of complex protein mixtures. Given that the expression of genes and gene products can differ between tissues and that this expression can be influenced by a variety of physiological signals, both stimulatory and inhibitory, SILAC can be a useful tool for monitoring protein synthesis, turnover, and degradation under various physiological and experimental circumstances. Additionally, a temporal tracking of protein abundance can be facilitated by the use of various isotopic variants of an amino acid.